Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: 4D electron microscopy of T cell activation

doi: 10.1073/pnas.1914078116

Figure Lengend Snippet: Non-antigen-specific T cell activation. (A) Plot of PINEM intensity of ionomycin-treated Jurkat T cells with increasing concentration of ionomycin. Error bars represent ± SEM (n = 8 to 12 cells). (B) Bar plot of IL-2 secretion by ionomycin-treated Jurkat cells measured by ELISA. Top error bars represent s.d. (n = 3). (C) Corresponding confocal fluorescence micrographs of filamentous actin. (Scale bar: 20 μm.) (D) Plot of zeta potential of ionomycin-treated Jurkat cells. Error bars represent ± SEM (n = 3). (E) Correlation analysis of PINEM intensity (A) and zeta potential (D) (R2 = 0.62). Unless otherwise specified, PMA concentration is fixed at 50 ng⋅mL−1.

Article Snippet: CD28 monoclonal antibody (MAB342), human CCL4 ELISA kit (DMB00), and human IL-2 ELISA kit (D2050) were purchased from R & D Systems.

Techniques: Activation Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Fluorescence, Zeta Potential Analyzer

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: 4D electron microscopy of T cell activation

doi: 10.1073/pnas.1914078116

Figure Lengend Snippet: Antigen-specific T cell stimulation. (A) Plot of PINEM intensity (red) and MHC binding (blue) of Jurkat cells treated with increasing concentration of pMHC tetramer (nanomolar concentrations). PINEM intensity is shown as mean ± SEM, collected from 4 to 10 individual cells. MHC binding (surface fluorescence intensity per cell) is shown as mean±SEM (n = 2); each measurement is from 28,750 to 90,000 cells in individual wells. (B) Correlation analysis of A shows that PINEM intensity vs. MHC binding represents a strong correlation (R2 = 0.88). (C) Bar plot of IL-2 secretion by pMHC-treated Jurkat cells measured by ELISA. Top error bars represent SD (n = 3). (D) Corresponding confocal fluorescence micrographs of filamentous actin. (Scale bar: 20 μm.) Unless otherwise specified, anti-CD28 antibody concentration is fixed at 1 μg⋅mL−1.

Article Snippet: CD28 monoclonal antibody (MAB342), human CCL4 ELISA kit (DMB00), and human IL-2 ELISA kit (D2050) were purchased from R & D Systems.

Techniques: Cell Stimulation, Binding Assay, Concentration Assay, Fluorescence, Enzyme-linked Immunosorbent Assay

Journal: Chemical science

Article Title: Dual-targeting biomimetic delivery for anti-glioma activity via remodeling the tumor microenvironment and directing macrophage-mediated immunotherapy.

doi: 10.1039/c7sc04853j

Figure Lengend Snippet: Fig. 4 (A) The cytotoxicity test (IC50) in the U87 and GL261 cells. (B) The cytotoxicity test in the U87 cells cultured with M1-CM or M2-CM. (C) The expression of MRs in TAM1 and TAM2 and the polarization modulation of the drugs. (D) The re-education of TAM2 treated with drugs and the expression of MRs and B7-H4. The ELISA analysis of the expression of TNF-a (E) and TGF-b1 (F) in TAM1 and TAM2 after drug treatment. The ELISA analysis of the expression of TNF-a (G) and TGF-b1 (H) in TAM1 and TAM2 cocultured with U87 cells after treatment.

Article Snippet: The mouse IFN-g Elisa Kit was purchased from R&D Systems (USA).

Techniques: Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Life

Article Title: Effects of Physical Exercise and Motor Activity on Depression and Anxiety in Post-Mastectomy Pain Syndrome

doi: 10.3390/life14010077

Figure Lengend Snippet: Evaluation of the intensity of pain and BDNF, IL-17, IL1-β, ACTH, and cortisol in post-mastectomy pain (PMP) and non-post-mastectomy pain (non-PMP) groups at 3 and 6 months after surgery.

Article Snippet: The following kits were used: IL-17 (R&D System; Manufactured and Distributed by: USA R&D Systems, Inc. 614 McKinley Place NE, Minneapolis, MN 55413, USA, Catalog #: D1700); IL1-β (R&D System Catalog #: DLB50); BDNF (R&D System Catalog #: DBD00); ACTH (Novus biologicals; Novus Biologicals USA 10730 E. Briarwood Avenue Centennial, CO 80112, USA; NBP2-66401); and cortisol (Novus biologicals NBP3-18003).

Techniques:

Journal: Life

Article Title: Effects of Physical Exercise and Motor Activity on Depression and Anxiety in Post-Mastectomy Pain Syndrome

doi: 10.3390/life14010077

Figure Lengend Snippet: Evaluation of the intensity of pain, and biomarkers of BDNF, IL-17, IL1-β, ACTH, and cortisol in the DA-PM subgroup and non DA-PMP subgroup at 3 and 6 months after surgery.

Article Snippet: The following kits were used: IL-17 (R&D System; Manufactured and Distributed by: USA R&D Systems, Inc. 614 McKinley Place NE, Minneapolis, MN 55413, USA, Catalog #: D1700); IL1-β (R&D System Catalog #: DLB50); BDNF (R&D System Catalog #: DBD00); ACTH (Novus biologicals; Novus Biologicals USA 10730 E. Briarwood Avenue Centennial, CO 80112, USA; NBP2-66401); and cortisol (Novus biologicals NBP3-18003).

Techniques:

Journal: Life

Article Title: Effects of Physical Exercise and Motor Activity on Depression and Anxiety in Post-Mastectomy Pain Syndrome

doi: 10.3390/life14010077

Figure Lengend Snippet: Evaluation of the intensity of pain, and BDNF, IL-17, IL1-β, ACTH, and cortisol levels in the DA-PMP subgroup at 3 and 6 months after surgery, according to the physical activity score.

Article Snippet: The following kits were used: IL-17 (R&D System; Manufactured and Distributed by: USA R&D Systems, Inc. 614 McKinley Place NE, Minneapolis, MN 55413, USA, Catalog #: D1700); IL1-β (R&D System Catalog #: DLB50); BDNF (R&D System Catalog #: DBD00); ACTH (Novus biologicals; Novus Biologicals USA 10730 E. Briarwood Avenue Centennial, CO 80112, USA; NBP2-66401); and cortisol (Novus biologicals NBP3-18003).

Techniques: Activity Assay

Journal: Clinical Epigenetics

Article Title: The immunomodulatory anticancer agent, RRx-001, induces an interferon response through epigenetic induction of viral mimicry

doi: 10.1186/s13148-017-0312-z

Figure Lengend Snippet: RRx-001 induced IFN response through upregulation of type I and III IFN expression and JAK/STAT pathway. Cells were transiently (24 h) treated with 0.5 μM RRx-001 or 0.5 μM 5-AZA and subsequently maintained in drug-free medium for an additional 7 days. RRx-001 induced a significant increase in type I IFN (IFN-β) ( a ) and type III IFN (IL-29/IL-28B) ( b ) secretion into culture medium by HCT 116 cells as measured by ELISA. Transcript levels of IL29 / IL28A were also increased as determined by qPCR ( c ). ISGs ( IFI27 , IFi44 , IFI44L , and IFI6 ) were upregulated by 5-AZA and RRx-001 but blocked by the JAK/STAT inhibitor ruxolitinib (rux) at 2 μM concentration ( d ). Expression of ISGs ( IRF7 , ISG15 , DDX58 , and OASL ) was also upregulated in HCT 116 cells cultured in conditioned medium containing secreted IFNs induced by 5-AZA and RRx-001 as determined by qPCR ( e )

Article Snippet: Levels of type I IFN (IFN-β) and type III IFN (IL-29/IL-28B) were determined using a VeriKine Human IFN-beta ELISA Kit (PBL Assay Science, Piscataway Township, NJ, USA) and a Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA Kit (R&D Systems, Minneapolis, MN, USA) according to manufactures’ instructions, respectively.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay, Cell Culture

Journal: Cells

Article Title: Overexpression and Activation of αvβ3 Integrin Differentially Affects TGFβ2 Signaling in Human Trabecular Meshwork Cells

doi: 10.3390/cells10081923

Figure Lengend Snippet: Differentially expressed genes in WTβ3 and/or CAβ3 cells that are associated with glaucoma. Except where noted by *, Log 2 FC values were reported in DGE analysis using EdgeR. * Genes that were filtered out due to low abundance did not originally have a FC determined (ND). Log 2 FC was calculated from the raw count data. The values in parentheses were originally calculated using EdgeR.

Article Snippet: ELISA analysis was performed using an R&D Systems Human TGF-beta 2 Quantikine ELISA Kit (R & D Systems, Minneapolis, MN), and the procedure was performed according to the manufacturer’s instructions.

Techniques: Migration, Activity Assay, Binding Assay, Ubiquitin Proteomics

Journal: Cells

Article Title: Overexpression and Activation of αvβ3 Integrin Differentially Affects TGFβ2 Signaling in Human Trabecular Meshwork Cells

doi: 10.3390/cells10081923

Figure Lengend Snippet: Differentially expressed genes in WTβ3 and/or CAβ3 cells that are associated with IOP regulation. Log 2 FC values were reported in DGE analysis using EdgeR.

Article Snippet: ELISA analysis was performed using an R&D Systems Human TGF-beta 2 Quantikine ELISA Kit (R & D Systems, Minneapolis, MN), and the procedure was performed according to the manufacturer’s instructions.

Techniques: